The temperature in the plate chamber is ambient and isothermal. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. This document is provided to customers who have purchased Molecular Devices, Inc. Optimize. and the assembly was incubated for 5 minutes in the dark prior to reading the plate. , call 1-800-635-5577. Learn how Molecular Devices FlexStation 3 Multi-Mode Microplate Reader provides life science industry solutions. Measure more accurately. edu. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Examples include ELISAs for quantitation of cytokines and protein concentration determination using the Bradford assay. Learn how you can utilize microplate readers to drive landmark discoveries. Wait 20 seconds. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Cet appareil ISM est confomre à la norme NMB-001 du Canada. The adapter is required for optimum performa nce with standard 96-well and 384-. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Consider non binding surface type plate (Greiner and Corning are 2 manufacturers)Molecular Devices microplate readers. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. Title: F3/F5 Multi Mode Microplate Readers | Molecular Devices Author: Molecular Devices, LLC Subject: Learn how the F3/F5 Multi-mode microplate readers can utilize its filter sets to accurately perform measurements with up to five modes of detection. Experimental details and customization of the group table. The advantage of using labeled probes is detection specificity. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Turn the reader off by pulling the power cord. 0 . This multi plate reader is excellent to work with for checking the U. , target CV per well = 1. absorbance plate readers. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. Shop now. Flexibility to read a variety of plate formats. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:Chapter 1: Introduction The SpectraMax iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. 0%, raw data units = counts/sec. A). The Back Panel Figure 1. Molecular Devices offers a broad range of technologies for high-throughput screening, genomic and cellular analysis, colony selection, and microplate detection. Clone Screening. Published Dec 16, 2020 | Updated Dec 16, 2020. With optimized reagents, option to operate the. Search. 5. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. The plate reader optics are tunable for absorbance. FlexStation™ II Systems provide integrated fluid transfer capability from a source plate or reservoir to an assay plate with concurrent kinetic fluorometric analysis of reactions. Sale price $3,495. This offer includes SpectraMax i3x, SpectraMax. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. These systems are hybrids that allow you to choose between. ” So, acquisition and analysis come from one software system. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. SpectraMax Microplate Readers. Set up Identify Acquire Analyze Imaging with the SpectraMax MiniMax Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3 System. Microscopes and Microplate Instrumentation. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Select Start>Control panel>Device Manager. Gregory et al. 997 (n = 3). What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Instrument settings can be saved as a protocol file. Page 1 of 8 . SpectraMax® Microplate Readers and SoftMax® Pro Software. 1 up to 3600 sec. 0 × 103 to 2. 5 µL of each sample was pipetted into a 96-well. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. In . First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Scanning options are endpoint, kinetic, spectral. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Users of Molecular Devices' SpectraMax® i3 Multi-mode reader can even add an optional. The Back Panel Figure 1. Next generation SpectraMax® absorbance microplate readers. To contact technical support by phone in the U. SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. 00 USD. The PyroGene assay has been optimized to be linear from 0. Item SpectraMax i3x Multi-Mode Microplate Reader. Endpoint readers are prolific in the laboratory since absorbance has become the detection of choice for many applications. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. , attenuation mode = out. Restart the system. Molecular Devices makes. QuickSync. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. The instrument will read theThe FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. After reading the ELISA plate on both readers, each standard curve was nearly identical. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. 1. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Plus 384. Data collection speed is sufficient for running fast cell-based kinetic assays. SoftMax Pro 7. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and. APP Automated. The. This process should take about 3 - 5 minutes. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. MycoAlert Substrate was then added to the wells, and the plate was incubated for 10 minutes at room temperature. Figure 1. 1. Important things to consider when purchasing a multimode microplate reader. In an analogous manner, the same sample names were assigned to a CuvetteSet section. As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Easy-to-use touchscreen allows you to quickly set up your experiments, use preconfigured analysis methods, and view tutorial videos. 1. The SpectraMax M2 and M2e are versatile and reliable. Molecular Devices' microplate readers. Absorbance Plate Reader Comparison Chart. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. We would like to show you a description here but the site won’t allow us. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Price Get Quote. 0. We therefore gathered salient. Retrieving Each. HTRF-compatible SpectraMax Multi-Mode Readers. 1-1. 2. The direct transfer capability reduces We would like to show you a description here but the site won’t allow us. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. The computer controlling the plate reader runs Windows XP. From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. For a higher . Read plate standard and mix. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more ( Molecular Devices ) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Endpoint. High-precision 9 channel system; filter-less with up to 6 wavelengths. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. (Molecular Devices cat. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Instrument settings can be saved as a protocol file. Test up to 3. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. 13. Step 3: Click “Settings” and a screen will appear (Figure 1). The Molecular Devices FilterMax F5 (replacing our old Analyst plate reader) is a versatile instrument that offers detection based on fluorescence, fluorescence polarization, luminescence, absorbance, and time resolved FRET. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Find our technical support information along with contact details. PURPOSE. SpectraDrop High-Throughput Screening (HTS) Kit. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. The sample is typically placed in a cuvette through which light is sent horizontally. 5: Schematic of the back panel of a reader. Microplate Reader Shelf. Read height should be optimized for Vmax® plate reader specifications. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. Multimode microplate readers deliver both performance and economy. 2. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. These tools can be used in conjunction with SoftMax® Pro Software validation package and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. In addition, users can define individual reagents and concentrations to be delivered to each well. Introduction. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Plate section without PathCheck applied. Maintaining data integrity is a. Microplate Reader Shelf. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. Step 1: Capture antibody binds to ELISA plate wells. SoftMax® Pro Software Version 5 for Mac and Windows User Guide 5000938 B June 2010 SoftMaxPro_UserGuide_5000938. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Molecular DevicesThe Marker Expression Protocol is used to identify cell areas labeled with the fluorescein-conjugated anti-VCAM antibody. The SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. One plate carrier adapter is provided with the instrument. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. Methods. Check software compatibility with Windows 10 and locate your software installer. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell. 2. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Description: ScanLater* Western Blot Detection Cartridge, enables the first western blot detection capability to a microplate reader, Time-resolved fluorescence detection, Xenon flash lamp excitation source. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader ( Molecular Devices, San Jose, CA, USA) at 30 °C. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. FLIPR. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Due to their ability to simultaneously measure multiple wells in a single run, fluorescence plate readers are useful for conducting various assays, such as quantitative assays, enzyme assays, and ELISAs. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. The computer controlling the plate reader runs Windows XP. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. S. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. The automated ELISA workflow has two positive outcomes. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. tools to expand the boundaries of their research. Plate stacker and robot integration The SpectraMax 340PC384 reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. For biochemical assays, the washer removes unbound material and unreacted reagents. 1). Plate reader instructions Computer. Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. An industry-renowned instrument for monitoring GPCRs and ion channels. PURPOSE. US HEADQUARTERS. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. (SPR) were monitored by Clone Select Imager (CSI, Molecular Devices, San Jose, CA, USA) and by ELISA assay according to the protocol in Brezinksy etFLIPR Tetra fluorometric imaging plate reader (Molecular Devices, Inc) with EMCCD Camera and 440–480 nm and 515–575 nm emission filters; Methods Cell Preparation. For SpectraMax ABS Plus reader, r 2 = 0. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Samples with volumes as low as 2 µL can be measured. The FlexStation 3 Reader combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. (Figure 1). 5. Step 4: Incubate at room temperature for 1 hour, protected from light. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Description Figure 1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Superior optical system includes a xenon flash lamp and features. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with allSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Avoid losing high signal. 304 signal level saturation. The adapter is required for optimum performance with standard 96-well and 384-. 20-plate magazine (pair) 0200-6016. Register to download your free eBook today. View the Molecular Devices website. Are you looking for a versatile and affordable microplate reader that can perform various assays and measurements? Check out the FilterMax F3 and F5 Multi-Mode Microplate Readers, which offer absorbance, fluorescence, luminescence, and more read modes with high performance and reliability. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). #4TI-0655) Microplates. The guide also covers the software features, the specifications, and the accessories of the systems. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular. Lamp source is a Xenon flash lamp (1 Joule/flash). 0. If the computer is connected to the Internet: Select Search Automatically for updated driver software. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Instrument settings can be saved as a protocol file. Latest Citations: For a complete list, please click here. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. The two different data import options are tailored to quickly get your data into SoftMax Pro to provide a single source of software for all your data reduction needs. 305 reference level saturation. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Perform plate-based assays on a large number of plates without having to manually feed them into your microplate washer and reader. Expanded wavelength coverageOther readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Description Figure 1. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. With optimized reagents, option to operate the. The FLIPRTETRA, which debuts today at. 0. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. $2,800. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. To update the driver. (1) Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19 Virulence December 31, 2022 David J. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. 340 PC384. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The instruments feature a bottom-read mode ideal for whole-cell-based assays, and may beSoftMax Pro GxP Software. Tests every subsystem of the reader . It was then read from the bottom on a SpectraMax ® i3 Multi-Mode Microplate Reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Plate reader. First, as mentioned before, the walkaway time significantly. Multi-mode Plate Reader. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. The Synergy H4 is a convenient benchtop microplate reader that offers robust. with the kit reagent and transferred to the plate reader for evaluation at the same time. S. Note that this procedure is only for the SpectraMax L model. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. Step 2: Launch SoftMax Pro Software. 0 . Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. FlexIII. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 5 µL of each sample was pipetted into a 96-well. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. This reader's dual monochromators for. 005 EU/mL to 5. Imaging with the MiniMax 300 Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Step 1: Turn on your microplate reader. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. The SpectraMax 190 reader can be integrated with Molecular . SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The plate is set up for reading and images are acquired according to specified parameters. One plate carrier adapter is provided with the instrument. 00. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. July 17, 2023. The instrument supports the following read modes:. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. In the M2, M5 and Plus. 9. Plate Formats: 6 to 384-well microplates. Molecular Devices. Page 1 of 8 . Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Firmly grasp the snap connector beside the snap, then push the snap down. Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast,. Fluorescence Microplate Reader Comparison Chart. Use a Phillips screwdriver to remove the screws, and then remove the plate from the instrument. The lower limit of detection (LLD) was defined as the level of. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. Recombinant protein expression Nucleocapsid. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. 1. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Research-grade results. 3860 N First Street San Jose, CA 95134. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. and it is unrealistic to expect one experiment to occupy a plate reader for multiple hours or days. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Flexibility to read a variety of plate formats. Label-free detection. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. was added and absorbance was measured at 450 nm and 650 nm using a plate reader (Molecular Devices, SpectraMax ABS Plus, SoftMax Pro 7. Under "Category", select "Plate Type". The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Molecular Devices products are warranted to meet the stated specifications. 1 - 16 of 69. Learn how Molecular Device's VersaMax Microplate Reader can be combined with the Pyrochrome Assay from Associates of Cape Cod, Inc. This process should take about 3 - 5 minutes. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Learn how you can utilize microplate readers to drive landmark discoveries. Find regional support. . IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 6 kg (30 lbs. 1 up to 3600 sec. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 5032018C 8 Chapter1:Introduction TheEMax® PlusMicroplateReaderisafilter-basedabsorbancemicroplatereaderwitheight standardfiltersforawiderangeofapplications. Learn more about their features and specifications in. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Microplate Reader Comparison Charts Published Dec 16, 2020 | Updated Dec 16, 2020 Products : General, FilterMax F5 reader Please click on the links below to. M5. Molecular Devices' microplate readers. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. 2. For nearly 30 years, Molecular Devices has provided scientists with . 0 EU/mL, but the additional standard concentration (0. From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. This five-mode microplate reader with automatic filter identification and western blot capability is the complete laboratory solution to help scientists increase. 4: Test tube cover. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 0. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Published Dec 16, 2020 | Updated May 19, 2023. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 190. Image courtesy of Molecular Devices. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre.